A Melting Temperature Calculator
Latest version: 2.0.1, updated at 2022-02-14
Usage
Input sequences (up to 6, case-insensitive) in “Primer Sequence” entries, and click “Calculate”.
The GC% and Tm calculated with Nearest neighbor, Wallace and GC% methods would be displayed in a new window.
Modify Parameters
The default concentrations are 50 mM of salt and 500 nM of primer. Users can modify these concentration before clicking the “Calculate” button. Please be noticed that, only integer is acceptable for the concentration value.
Download
Executable file for macOS could be download from here.
LICENSE
Tm.Calc is following MIT license. The LICENSE file could be found along with Tm.Calc source code.
MIT License
Copyright (c) 2021 Zhe Wang
Permission is hereby granted, free of charge, to any person obtaining
a copy of this software and associated documentation files (the "Sof-
tware"), to deal in the Software without restriction, including with-
out limitation the rights to use, copy, modify, merge, publish, dist-
ribute, sublicense, and/or sell copies of the Software, and to permit
persons to whom the Software is furnished to do so, subject to the f-
ollowing conditions:
The above copyright notice and this permission notice shall be inclu-
ded in all copies or substantial portions of the Software.
THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPR-
ESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCH-
ANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN
NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CL-
AIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, T-
ORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOF-
TWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE.